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How Viruses Enter Cells, Replicate, and Evade Immunity

1Viral Architecture and the Logic of the Life Cycle2Attachment and Entry: Crossing the Membrane3Genome Replication and Gene Expression4Assembly, Egress, and Transmission5Innate Immune Detection of Viral Infection6Adaptive Immunity: Antiviral Antibodies and T Cells7Immune Evasion Strategies8From Mechanism to Intervention: Antivirals and Vaccines
Assembly, Egress, and Transmission

Selective Genome Packaging into New Capsids

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Look at the diagram as a sorting problem. On the left, viral genomes carry a packaging signal, drawn as a short colored tag. On the right, host RNA has no tag. The capsid protein only binds the tagged genome, so assembly starts there and builds a shell around it. Host RNA is left outside. That is why most particles that form contain a viral genome rather than a random piece of cellular RNA.
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Assembly is not random. A capsid protein that simply polymerized around whatever nucleic acid was nearby would produce mostly empty shells or shells filled with host RNA, wasting the structural proteins the virus worked hard to make. Selective packaging solves this by coupling capsid assembly to recognition of a packaging signal, a short sequence or folded structure in the viral genome that is bound by a viral structural protein, usually the capsid protein itself or a dedicated packaging protein.

The logic is a hand-in-glove fit. The packaging signal is present only on the viral genome, so when the structural protein binds it, the resulting nucleoprotein complex becomes the nucleus for further capsid assembly. Genomes that lack the signal, including host mRNAs, are not recruited. In many RNA viruses the same protein that recognizes the signal also forms the capsid, so recognition and assembly are physically linked. In DNA viruses the genome is often inserted into a preformed procapsid through a portal, a dedicated channel that uses ATP to thread the DNA inside.

The consequence is that a productive infection yields mostly full, genome-containing particles. The fraction of empty particles that does appear is a byproduct of assembly that ran without a genome, not the intended product, and it is usually non-infectious because it cannot deliver a genome.

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